ABSTRACT
Objective:To investigate the effect of body axis rotation combined with rhythmic breathing training on lower limb motor function and balance ability of stroke patients with hemiplegia. Methods:From June, 2018 to June, 2020, 50 patients with hemiplegia after stroke from Physical Therapy Department of Rehabilitation Hospital Affiliated to Fujian University of Traditional Chinese Medicine were divided into control group (n = 25) and experimental group (n = 25). Both groups accepted routine rehabilitation, while the experimental group accepted body axis rotation and rhythmic breathing training for core muscle strength in addition, for six weeks. The total training time was the same for both groups. They were assessed with Fugl-Meyer Assessment-Lower Extremities (FMA-LE), Berg Balance Scale (BBS), Timed 'Up & Go' Test (TUGT) and 10-meter walking speed (10MWS) before and after treatment. Results:The scores of FMA-LE and BBS, and 10MWS increased in both groups after treatment (|Z| > 4.375, P < 0.001), and increased more in the experimental group than in the control group (|Z| > 3.415, P < 0.01); while the time of TUGT decreased in both groups (Z = -4.372, P < 0.001), and decreased more in the experimental group than in the control group (Z = -2.804, P < 0.01). Conclusion:Body axis rotation combined with rhythmic breathing training for core muscle strength may promote the recovery of lower limb motor function and balance ability for stroke patients with hemiplegia.
ABSTRACT
Objective To explore the outcome of brain derived neurotrophic factor(BDNF) and parvalbumin (PV) positive neurons after hyperbaric oxygen intervention for the valproic acid(VPA) autism rats in hippocampal CA1 region.Methods The animal model of autism was established by the methods of Schneider and Przewlocki.Animal model groups of autism were obtained in male offspring of the Wistar rats that received intraperitoneal injection of 600 mg/kg VPA on the 12.5 day after pregnancy according to the eye opening time,behavior,weaning weight and the learning and memory abilities which were evaluated by the Y electricity maze test on the 28th day after birth.Forty-eight male VPA autism rats were randomly divided into high-pressure high-oxygen group,high-pressure air group and normal pressure high-oxygen group and normal pressure air group(n =12,each group).Normal control group was obtained in male offspring of Wistar rats that received intraperitoneal injection of equivalent physiological saline in the same period (n =12).Using the immunohistochemistry methods and image analysis to examine the number of BDNF and PV positive neurons in hippocampal CA1 region of different groups.Results The number of BDNF positive neurons in normal pressure air model group was more than that in the normal pressure air control group,with statistical difference between them(5.00 ± 1.60 vs 3.00 ± 1.04,t =3.633,P =0.001).The number of the PV positive neurons in the normal pressure air model group was more than that of the normal pressure air control group,with statistical difference between them (5.33 ± 0.99 vs 2.83 ± 1.29,t =5.369,P =0.000).The number of the PV positive neurons in the high-pressure high-oxygen group was less than that in the normal pressure air model group,with statistical difference between them (3.33 ±0.99 vs 5.33 0.99,t =4.975,P =0.000).The number of the PV positive neurons in the high-pressure highoxygen group was less than that in the high-pressure-air model group (3.33 ± 0.99 vs 4.67 ± 1.92,t =-2.138,P =0.044).Conclusions The pathogenesis of autism may be related to the expression level of BDNF and PV in the hippocampal CA1 region and this may serve as the mechanism of hyperbaric oxygen intervention of autism animal model for effective treatment.
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The purpose of this paper is to clarify the structure-activity relationship of anti-tumor activity of diosgenin derivatives in vitro. Study has found that diosgenin can inhibit the reproduction of tumor cells by inducing apoptosis and the main target spot of this effect is Bcl-2. Based on the characteristics of pharmacophoric points' of the three-dimensional pharmacophore for Bcl-2 inhibitors, we have docked lots of diosgenin derivatives with Bcl-2, then synthesized 31 compounds of them, finally assessed the anti-tumor activity of the diosgenin derivatives in vitro against A375, A549, HepG-2 and K562. Preliminary studies of SAR have indicated that the aliphatic esters, and aromatic esters of diosgenin without F ring have no anti-tumor activity in vitro. The triazole bromides of diosgenin all achieve fairly good anti-tumor activity in vitro, and those with larger hydrophobic group have the better activity. The stronger is the hydrogen bonding interaction and dipole-dipole interaction of the heterocyclic of diosgenin and diosgenin without F ring and the acid ester of diosgenin without F ring, the better is the activity of derivatives.